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. 2018 Apr 12;2018:2021645. doi: 10.1155/2018/2021645

Figure 3.

Figure 3

Hypoxia-induced HIF-1α stabilization is dependent in part on HO-2 and mitochondrial ROS. (a) HIF-1α protein levels are increased in hepatocytes following 90 minutes of hypoxia. (b) Inhibition of HO activity with tin protoporphyrin (SnPP) limits hypoxia-induced increases in HIF-1α protein levels. (c–f) HO-2 siRNA decreases hypoxia induces HIF immunocytochemistry (d) and 1-α levels as determined by Western blotting (c) and luciferase-reporter assay (f). Furthermore, inhibition of mitochondrial ROS production by MitoTEMPO also decreases HIF-1α as determined by immunocytochemistry (e) and luciferase-reporter assay (f). ∗P < 0.05 compared to control siRNA 21% O2; #P < 0.05 compared to control siRNA 1% O2.