Figure 4.

Hypoxia-induced JNK phosphorylation is partially dependent on HO-2 and mitochondrial ROS production. (a) Phosphorylated JNK (p-JNK) is increased in hepatocytes following 90 minutes of hypoxia. (b) Inhibition of HO activity with tin protoporphyrin (SnPP) limits hypoxia-induced increases in phosphorylation of JNK. (c) HO-2 siRNA limits phosphorylation of JNK during a one-hour time course of hypoxia treatment. (d) Treatment with MitoTEMPO to limit mitochondrial ROS generation decreases hypoxia-induced JNK phosphorylation at a 60-minute time point.