Table II.
Pigment content of the f2 mutant after development under various growth regimes
| Pigment | 20/250 | 20/800 | 5/50 | 5/250 |
|---|---|---|---|---|
| μg g−1 fresh wt | ||||
| Chl a/b | >20 | >20 | >20 | >20 |
| Neoxanthin | 3.9 ± 0.5 | 4.4 ± 0.4 | nda | 3.5 ± 0.5 |
| Lutein | 59 ± 3 | 64 ± 5 | 38 ± 3 | 89 ± 3 |
| β-Carotene | 77 ± 4 | 70 ± 3 | 44 ± 2 | 44 ± 2 |
| Violaxanthin | 40 ± 2 (100 ± 2) | 28 ± 2 (53 ± 2) | 25 ± 2 (100 ± 2) | 68 ± 4 (90 ± 1) |
| Antheraxanthin | nd (nd) | 7.9 ± 1.0 (15 ± 1) | nd (nd) | 6.1 ± 0.4 (5 ± 1) |
| Zeaxanthin | nd (nd) | 16.8 ± 1.5 (32 ± 2) | nd (nd) | 4.5 ± 0.3 (5 ± 1) |
| V+A+Z | 40 ± 2 | 53 ± 2 | 25 ± 2 | 75 ± 4 |
Pigments were separated and quantified by HPLC, as described in Methods. The results are presented as means ± sd of three replicate measurements from three different plants. Numbers in parentheses represent the percentages of the total xanthophyll-pool size (V+A+Z).
a
nd, Not determined.