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JNK inhibitor SP600125 did not inhibit the activation of TLR4 by persistent or intermittent high glucose. THP-1 cells were pretreated with SP600125 (20 μM) for 2 h followed by stimulation with intermittent high glucose, persistent high glucose, or LPS. Then the expression of TLR4 was examined. ∗∗p < 0.01, ∗∗∗p < 0.001 (n = 4 for each group).
