Figure 3.
Effects of vitamin D treatment on Pin1 protein expression and activity of high glucose-cultured HUVECs. HUVECs were inoculated in 6-well plates, cultured with 5% FBS at 70%~80% confluence for 24 h, and then coincubated with high glucose (33 mM) and vitamin D (10−8~10−6 M) for 72 h. Total proteins were extracted after immunoblotting analysis. Pin1 protein expression levels of HUVECs were expressed as the ratio of Pin1 over β-actin. Pin1 activity of HUVEC lysate was measured by using a commercially available kit. (a) Effects of vitamin D treatment on Pin1 protein expression of high glucose-cultured HUVECs; (b) effects of vitamin D treatment on Pin1 protein activity of high glucose-cultured HUVECs. HG: high glucose (33 mM); results are presented as mean ± SEM (n = 5); ∗ P < 0.05 versus control; # P < 0.05 versus HG (33 mM); + P < 0.05 versus HG (33 mM) + VitD (10−7 M).