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. 2018 Apr 15;2018:9416391. doi: 10.1155/2018/9416391

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Copyright © 2018 Yingdi Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The proportion of total CD4⁺CD25⁺Foxp3⁺ Tregs cells in the peripheral blood and spleen. (a) The frequency of Treg cells in the blood (n = 6–9/group) (b) and in the spleen (n = 6–9/group). (c) The number of Foxp3⁺ cells in colonic tissue (the white arrows indicate Fox3⁺ cells). (d) Western blot analysis of mice colonic Foxp3 protein expression level in each group. Group 1 normal (n = 3); group 2 Bifico (n = 6); group 3 colitis (n = 6); group 4 pretreated-Bifico-colitis (n = 6); group 5 pretreated + treated-Bifico-colitis (n = 6). Data represent means ± SEM. ^∗P < 0.05 and ^∗∗∗P < 0.001.