Table 2.
Proposed chuangxinmycin biosynthetic gene cluster and gene functions.
| Gene | cxn name | Size (aa) | Protein homologue | Identity/Similarity (100%) | Proposed function |
|---|---|---|---|---|---|
| ats3059 | 264 | ThiG, thiazole synthase (WP_055570175.1), Streptomyces atriruber | 94/96 | Thiazole synthase | |
| ats4175 | 331 | Hypothetical protein (WP_047121450.1), Streptomyces leeuwenhoekii | 55/63 | HTH_XRE family transcriptional regulator | |
| ats4176 | 346 | Peptidoglycan-binding protein (WP_044378158.1), Streptomyces cyaneogriseus | 72/83 | Peptidoglycan-binding protein | |
| ats4177 | 240 | Hypothetical protein (WP_086718244.1), Streptomyces angustmyceticus | 81/85 | Associated to methylation | |
| ats4178 | cxnA | 623 | Maturation radical SAM protein 1 (WP_063754376.1 RiPP), Streptomyces sp. NRRL S-1813 | 93/96 | Radical SAM protein, methylation |
| ats4179 | cxnB | 362 | ThnJ, aminotransferase (AMR44310.1), Streptomyces sp. FXJ1.172 | 52/63 | Aminotransferase |
| ats4180 | cxnC | 318 | 2-Dehydropantoate 2-reductase (WP_030988196.1), Streptomyces sp. NRRL S-1813 | 87/90 | Reductase |
| ats4182 | cxnD | 404 | ThnC, P450 (AMR44303.1), Streptomyces sp. FXJ1.172 | 37/48 | Cytochrome P450 |
| ats4181 | cxnE | 100 | MoaD family protein (KRT67113.1), Candidatus Dadabacteria CSP1-2 | 43/71 | Sulfur-carrier protein |
| ats4183 | cxnF | 238 | ThnF, hypothetical protein (AMR44306.1), Streptomyces sp. FXJ1.172 | 44/61 | JAMM (JAB1/MPN/Mov34) metalloprotease |
| ats4184 | cxnT | 496 | MFS transporter (WP_051818417.1), Streptomyces sp. NRRL S-1813 | 86/90 | Transporter |
| ats4185 | cxnR | 313 | LysR family transcriptional regulator (AJK58337.1), Amycolatopsis lurida NRRL 2430 | 37/50 | Pathway-specific regulator |
| ats4186 | trpRS | 300 | Ind0, tryptophanyl-tRNA synthetase (AJT38681.1), Streptomyces griseus subsp. griseus | 73/83 | Tryptophan-tRNA synthetase |
| ats4187 | 846 | Membrane protein (ANZ15560.1), Streptomyces noursei ATCC 11455 | 39/52 | Membrane protein | |
| ats4188 | 548 | Sodium/proline symporter (WP_037838132.1), Streptomyces sp. NRRL S-337 | 68/81 | Transporter | |
| ats4190 | 79 | No homologous protein | Unknown | ||
| ats6133 | 392 | Adenylyltransferase/sulfurtransferase MoeZ (WP_067275253.1), Streptomyces jeddahensis | 98/98 | SCP-activating enzyme |