Depletion of total cellular ATP in tobacco cell
cultures after treatment with SA or its analogs. A, Tobacco cell
cultures were incubated with various concentrations of SA. At indicated
time points, cell samples were taken for determining total cellular ATP
levels. B, Tobacco cell cultures were incubated with 0.5 mm
SA, acetylsalicylic acid (ASA), 5-chlorosalicylic acid (5-CSA),
3-hydroxybenzoic acid (3-HBA), or 4-hydroxybenzoic acid (4-HBA). ATP
levels were determined after 6 h of incubation and reported
(+se calculated from three independent assays). FW, Fresh
weight.