Effect of Tris DBA on MM proliferation. (A) The effect of Tris DBA (0–10 μM) on proliferation of MM.1S, MM.1R, H929, RPMI8826, and OPM2 cells for 24 h analyzed by MTT (i), and IC50 for each cell line is presented in the Table (ii). (B) The effect Tris DBA (0–10 μM) for 24 h on proliferation of normal PBMCs. (C) The effect of treatment with Tris DBA (0, 2 μM) for 24 h on proliferation signaling, S6R, pS6R, ERK1/2, and pERK1/2 in MM.1S cells by Western blotting (i), and quantification of phosphoprotein expression as ratio to total and normalized to no treatment by densitometry (ii). *p<0.05.