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. 2018 Apr 23;9:332. doi: 10.3389/fphar.2018.00332

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Copyright © 2018 Agarwal, Gratwohl, Cozad, Yang, Clancy and Harvey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The prostaglandin receptor (EPR) agonist PGE1 produces greater cAMP response in non-raft-associated membrane domains. (A–C) Representative time course of changes in the normalized FRET response (ΔR/R₀) in cells expressing Epac2-CAAX (CAAX, white circles), Epac2-MyrPalm (MyrPalm, red circles), and Epac2-camps (Epac2, blue circles), under control conditions and following exposure to 1 μM PGE1. Maximal response was elicited by subsequent exposure to 1 μM Iso plus 100 μM IBMX (Iso + IBMX). (D) Summary of average FRET responses to 1 μM PGE1 in cells expressing Epac2-camps (n/N = 5/3, blue bar), Epac2-CAAX (n/N = 5/3, white bar), and Epac2-MyrPalm (n/N = 6/3, red bar). Epac2-CAAX response to 1 μM PGE1 was significantly different from Epac2-camps and Epac2-MyrPalm responses (^∗p < 0.05, one-way ANOVA followed by Holm-Sidak method for pairwise multiple comparisons).