Figure 5.

Gel-filtration fractionation of npRNP sediments. A, Elution of [α-³²P]UTP and npRNPs (P100) from pre-induced tomato cells (top) and radiolabeled histone H4 mRNA in the absence (middle) and presence (bottom) of npRNPs. Bars, eluted radioactivity as percentage of total counts applied. Arrowhead, Elution of UTP in the absence of npRNPs. Solid line, A₂₈₀ in arbitrary units. Numbers below the graphs mark fractions of 10 mL. B, Fractions separated by SDS-PAGE and stained with Coomassie blue. Lane m, Marker. C, Immunoblots of fractions using antisera for Hsp70 and small Hsp (sHsp). Numbers in B and C correspond to the fraction numbers in A. Lanes c, Control (nonpolysomal sediment before gel filtration).