Gel-filtration fractionation of npRNP sediments.
A, Elution of [α-32P]UTP and npRNPs (P100) from
pre-induced tomato cells (top) and radiolabeled histone H4 mRNA in the
absence (middle) and presence (bottom) of npRNPs. Bars, eluted
radioactivity as percentage of total counts applied. Arrowhead, Elution
of UTP in the absence of npRNPs. Solid line,
A280 in arbitrary units. Numbers below the
graphs mark fractions of 10 mL. B, Fractions separated by SDS-PAGE and
stained with Coomassie blue. Lane m, Marker. C, Immunoblots of
fractions using antisera for Hsp70 and small Hsp (sHsp). Numbers in B
and C correspond to the fraction numbers in A. Lanes c, Control
(nonpolysomal sediment before gel filtration).