Fig. 3.

Different responses to potassium concentrations in the subiculum of the HS and No-HS groups. (a) Number (left) and amplitude (right) of events during 10-min LFP recordings in the presence of 6, 9, and 12 mM [K⁺]_o are normalized to those recorded in the presence of 3 mM [K⁺]_o. (b) Representative images of time-frequency power-spectral analysis of 10 averaged consecutive events with changes in [K⁺]_o. Note the ascending spots in HS. Scale bar: 100 ms. (c) Quantification of changing the frequencies at peak power in the presence of 3, 6, 9, and 12 mM [K⁺]_o. (a–c) In these studies, we used two slices per patient. (n) indicates the number of slices. *p < .05, Tukey-Kramer test after one-factor ANOVA. (d) Double immunofluorescence images of the subiculum indicating loss of Kir 4.1 immunoreactivity (red) in the processes of GFAP (green)-positive cells in HS. Scale bar: 50 μm. (e) Quantitative analysis of the total area of immunofluorescence. The immunofluorescence area of Kir4.1 was normalized to that of GFAP. *p < 0.05, Tukey-Kramer test after one-factor ANOVA. Lower panels are sample images of the original image (upper) and the extracted area for calculation (Red area: lower). Scale bars: 25 μm. Error bars, S.E.M.