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. 2018 Apr 23;9:854. doi: 10.3389/fimmu.2018.00854

Figure 4.

Figure 4

NF-E2-related factor 2 (Nrf2) deficiency leads to increased neutrophil infiltration in the lungs in response to respiratory syncytial virus (RSV) infection. Nrf2 KO and wild typr (WT) mice were infected with RSV or PBS and lungs collected at day 1 post-infection. (A) Neutrophils (CD11b+Ly6G+) recruitment to the lung by flow cytometry analysis. Imaging (B) and quantification (C) of lung neutrophils using the near infrared fluorescent imaging agent by intratracheal (i.t., upper panels) or intravenous tail injection (i.v., lower panels). Fluorescence intensity is represented in the images with a pseudocolor scale ranging from yellow (most intense) to dark red (least intense) and quantified in specific total radiant efficiency per lung region of efficiency, using the Living Image software. The bar graph represents mean ± SEM (n = 2–4 mice/group). **p < 0.01 when compared with WT/RSV mice.