FIGURE 1.

Targeting strategy for the generation of the Kir7.1-HA mice. (A) Scheme of the Kcnj13 gene showing its three exons (boxes) and the coding regions in gray. The guide (green) targets exon 2 in the genome and leads Cas9 to produce a double strand break upstream to the PAM sequence (underlined). The ssODN containing the HA tag sequence, the EcoRV site (in red) and the homologous arms (in black) is the template used for homologous directed repair (HDR). (B) Agarose gel showing the PCR products of WT and HA genomic DNA obtained using the primers shown in (A). The amplicon digested with EcoRV reveals the homozygous (HA) for the tag insertion.