Table 1.
Primer sequences used for quantitative real-time polymerase chain reaction (qRT-PCR) analysis
| Gene | Sequence |
|
|---|---|---|
| Forward(5′-3′) | Reverse(3′-5′) | |
| ALP | GAGATGGTATGGGCGTCTC | GTTGGTGTTGTACGTCTTGGA |
| Collα1 | GACATGTTCAGCTTTGTGGACCTC | GGGACCCTTAGGCCATTGTGTA |
| OPG | AGCTGCTGAAGCTGTGGAA | GGTTCGAGTGGCCGAGAT |
| RANKL | CGCTCTGTTCCTGTACTTTCGAGCG | TCGTGCTCCCTCCTTTCATCAGGTT |
| Runx2 | GCACAAACATGGCCAGATTCA | AAGCCATGGTGCCCGTTAG |
| Wnt10b | GGCTGTAACCACGACATGGAC | ACGTTCCATGGCATTTGCAC |
| Lrp5 | CACCATTGATTATGCCGACCAG | TGAGTCAGGCCAAACGGGTAG |
| β-catenin | ATTTGATGGAGTTGGACATGG | TGTTCTTGAGTGAAGGACTGA |
| GAPDH | CCA CAC CTT CTACAA TGA GC | TGA GGT AGT CAG TCAGGT C |
ALP, alkaline phosphatase; Collα1, type I collagen; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Lrp5, low density lipoprotein receptor related protein 5; OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor-B ligand; Runx2, runt-related transcription factor 2; Wnt, wingless-type MMTV integration site