FIG 1.

Flow chart of the PCR testing algorithm for the detection of Borrelia burgdorferi sensu lato DNA in CSF samples from children investigated for Lyme neuroborreliosis (LNB), with results. The two real-time PCR assays applied targeted the ospA and 16S rRNA genes. The total numbers and percentages (in parentheses) of children with various test outcomes are given in the boxes. *, all four children had >1 replicate positive for ospA, and no children had a positive result for the 16S rRNA gene only.