Single particle tracking (SPT) trajectories in freshly isolated H-2Dd compared to MHC−/− murine NK cells captured at 20 ms frame-to-frame rate. Each dot represents a single trajectory. (A–B) NKp46 microdomain median width: H-2Dd = 0.21, MHC−/− = 0.18 μm (A) and Ly49A microdomain median width: H-2Dd = 0.19 and MHC−/− = 0.24 μm (B). (C–D) NKp46 short range diffusion coefficient D1-10 median: H-2Dd = 1.1*10−3 and MHC−/− = 0.89*10−3 μm2/s (C) Ly49A short range diffusion coefficient D1-10 median: H-2Dd = 0.93*10−3 and MHC−/− = 1.5*10−3μm2/s (D). The tracks are the same as those depicted in Fig. 1. The boxes represent medians (line inside box), the 95 % confidence intervals (notches), first and third quartiles (end of boxes), and the whiskers 1.5 times the interquartile ranges. P-values: ** ≤ 0.01, *** ≤ 0.001 (two-sample Wilcoxon ranking test).