Table I.
Effects of bisphenol A on spindle morphology in bovine metaphase II oocytes.
| Ctrl | fM (10−15 M) | pM (10−12 M) | nM (10−9 M) | uM (10−6 M) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 10 | 100 | 1 | 10 | 100 | 1 | 10 | 100 | 50 | ||
| Bipolar | 175 (82.9) | 21(58.3) | 22 (48.9) | 60 (69.8) | 24 (77.4) | 27 (67.5) | 16 (80.0) | 26 (50.0) | 68 (54.8) | 37 (56.1) | 27 (48.2) |
| Bipolar abn | 26 (12.3) | 12 (33.3) | 19 (42.2) | 23 (26.7) | 7 (22.6) | 13 (32.5) | 4 (20.0) | 19 (36.5) | 41 (33.1) | 23 (34.8) | 24 (42.9) |
| Non-bipolar | 10 (4.7) | 3 (8.3) | 4 (8.9) | 3 (3.5) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 7 (13.5) | 15 (12.1) | 6 (9.1) | 5 (8.9) |
| P value | – | 0.0129 | <0.0001 | 0.0293 | 0.3216 | 0.0129 | 0.4074 | <0.0001 | <0.0001 | 0.0002 | <0.0001 |
The number of oocytes (percentage in parentheses) with bipolar spindles, bipolar spindles with abnormalities and non-bipolar spindles are shown from at least three independent experiments per concentration. P values < 0.05 indicate a significant difference compared to control as determined by Fisher’s exact test.