Table II.
Effects of bisphenol A on chromosome alignment in bovine metaphase II oocytes.
| Ctrl | fM (10−15 M) | pM (10−12 M) | nM (10−9 M) | uM (10−6 M) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 10 | 100 | 1 | 10 | 100 | 1 | 10 | 100 | 50 | ||
| Aligned | 190 (90.0) | 30 (83.3) | 33 (75.0) | 72 (83.7) | 27 (87.1) | 36 (90.0) | 19 (95.0) | 43 (82.7) | 85 (68.5) | 54 (81.8) | 39 (69.6) |
| Most aligned | 11 (5.2) | 2 (5.6) | 3 (6.8) | 4 (4.7) | 2 (6.5) | 2 (5.0) | 0 (0.0) | 3 (5.8) | 12 (9.7) | 3 (4.5) | 8 (14.3) |
| Dispersed | 10 (4.7) | 4 (11.1) | 8 (18.2) | 10 (11.6) | 2 (6.5) | 2 (5.0) | 1 (5.0) | 6 (11.5) | 27 (21.8) | 9 (13.6) | 9 (16.1) |
| P value | – | 1.0000 | 0.0434 | 0.5953 | 1.0000 | 1.0000 | 1.0000 | 0.8913 | <0.0001 | 0.3102 | 0.0044 |
The number of oocytes (percentage in parentheses) with aligned chromosomes, mostly aligned chromosomes and dispersed chromosomes are shown from at least three independent experiments per concentration. P values < 0.05 indicate a significant difference compared to control as determined by Fisher’s exact test.