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. 2018 Mar 9;293(17):6398–6409. doi: 10.1074/jbc.RA118.001879

Figure 3.

Figure 3.

Classic IL-11 signaling depends on the stalk length. A–G, equal amounts of the different Ba/F3-gp130 cell lines were stimulated with increasing amounts (0–100 ng/ml) of either IL-11 or Hy–IL-6 for 48 h. Concentration-dependent proliferation was determined as described in “Experimental Procedures.” The analyzed cell line is given above the respective diagram. Error bars represent S.D. of triplicates from one representative experiment. H, STAT3 phosphorylation of Ba/F3–gp130–IL-11RΔP343_L372 and Ba/F3–gp130–IL-11RΔQ333_L372 cells in response to 10 ng/ml IL-11 or 10 ng/ml Hy–IL-6 stimulation for 15 min was determined via Western blotting. Total STAT3 was detected to ensure equal protein loading. The ratio of pSTAT3/STAT3 was determined from three independent experiments.