Figure 7.

CgYapsins are required for colonization and dissemination to the brain in the mouse model of systemic candidiasis. A, kinetics of infection of C. glabrata WT and Cgyps1–11Δ cells in BALB/c mice. Mice were infected intravenously and sacrificed at the indicated days, and fungal burden in kidneys, liver, spleen, and brain was determined using a cfu-based assay. Diamonds, yeast cfu recovered from organs of the individual mouse; horizontal line, cfu geometric mean (n = 12–16) for each strain. Statistically significant differences in cfu between WT- and Cgyps1–11Δ-infected mice are marked (***, p < 0.001; ****, p < 0.0001; Mann–Whitney test). Of note, we could retrieve Cgyps1–11Δ cfu from the brain of only four mice of 14 infected animals 7 dpi. B, representative photomicrographs of hematoxylin and eosin– and PAS–stained kidney and brain sections from uninfected, WT-infected, and Cgyps1–11Δ–infected mice on day 3 after infection. Magnification was ×40. The arrowheads in tissue sections indicate PAS-positive yeast cells.