Table 3.
Plasmids used in the study
| Plasmid | Description | Reference |
|---|---|---|
| pRK74 | A CEN-ARS plasmid (pGRB2.2) of C. glabrata carrying S. cerevisiae URA3 as a selection marker. MCS sites are flanked by S. cerevisiae PGK1 promoter at one end and by 3′-UTR of HIS3 at the other end | Ref. 55 |
| pRK935 | CgYPS1 ORF cloned in the MCS of pRK74 | Ref. 9 |
| pRK936 | CgYPS7 ORF cloned in the MCS of pRK74 | Ref. 18 |
| pRK995 | A CEN-ARS plasmid (Addgene ID 45319) of C. glabrata carrying S. cerevisiae URA3 as a selection marker. MCS sites are flanked by S. cerevisiae promoter HHT2 at one end and by 3′-UTR of HIS3 at the other end | Ref. 56 |
| pRK1042 | CgYPS1D378A cloned in the MCS of pRK74 | This study |
| pRK1046 | CgYPS1D91A in the MCS of pRK74 | This study |
| pRK1370 | CgYPS1 cloned in the MCS of pRK995 | This study |
| pRK1372 | CgYPS2 cloned in the MCS of pRK995 | This study |
| pRK1374 | CgYPS4 cloned in the MCS of pRK995 | This study |
| pRK1376 | CgYPS5 cloned in the MCS of pRK995 | This study |
| pRK1378 | CgYPS8 cloned in the MCS of pRK995 | This study |
| pRK1380 | CgYPS9 cloned in the MCS of pRK995 | This study |
| pRK1382 | CgYPS10 cloned in the MCS of pRK995 | This study |
| pRK1384 | CgYPS11 cloned in the MCS of pRK995 | This study |
| pRK1388 | CgYPS3 cloned in the MCS of pRK995 | This study |
| pRK1407 | CgYPS6 cloned in the MCS of pRK995 | This study |
| pRK1418 | CgYPS7 cloned in the MCS of pRK995 | This study |