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. 2018 Mar 12;293(17):6497–6516. doi: 10.1074/jbc.M117.811190

Figure 7.

Figure 7.

Amino acid residues critical for noncanonical transglycosylase function are conserved in mycobacterial RodA. a, schematic depicting the organization of RodA across the membrane. Both the amino and carboxyl termini are predicted to be inside the cytoplasm with 12 transmembrane domains by TMHMM. Residues suggested to be critical for transglycosylase activity, Trp-175 and Asp-343, are marked. b, growth analysis of Msm, MsmΔr, MsmΔr::rMtb, MsmΔr::rMtb-W175R, MsmΔr::rMtb-D343R, and MsmΔr::rMtb-DM, streaked on 7H11 plates in the presence or absence of 1 μg/ml moenomycin. c, growth analysis of Msm, MsmΔr, MsmΔr::rMsm, MsmΔr::rMsm-W176R, MsmΔr::rMsm-D344R, and MsmΔr::rMsm-DM, streaked on 7H11 plates in the presence or absence of 1 μg/ml moenomycin. d, REMA assay for Msm, MsmΔr, MsmΔr::rMtb, MsmΔr::rMsm, MsmΔr::rMtb-W175R, MsmΔr::rMtb-D343R, MsmΔr::rMsm-W176R, MsmΔr::rMsm-D344R, and MsmΔr::rMtb-DM, and MsmΔr::rMsm-DM for isoniazid and moenomycin. The experiment was performed in triplicates, and a representative data set is shown.