Figure 2.

TLC analysis of the rac-GR24 hydrolysis by DAD2 in the presence of tolfenamic, mefenamic, and flufenamic acid. DAD2 was first incubated with a 4-fold molar excess of inhibitors for 30 min at 20 °C. GR24 was then added to a final 20-fold molar excess, and reactions were incubated at 25 °C. A positive control consisted of DAD2 (25 μm), GR24 (500 μm), 5% DMSO in PBS, and a negative control was GR24 (500 μm), 5% DMSO in PBS. Compounds were extracted after 3 and 16 h of incubation and analyzed by TLC.