Figure 3.

Yeast two-hybrid analysis of DAD2 interactions with downstream partners in the presence of inhibitors. Shown is inhibition of rac-GR24–induced DAD2/PhMAX2A (A) and DAD2/PhD53A (B) interactions by tolfenamic acid, mefenamic acid, and flufenamic acid. Protein-protein interactions are quantified by assaying β-gal activity in a yeast two-hybrid liquid culture system. Data points are the mean ± S.E. (error bars) of three technical replicates. *, GR24 treatments where the means are significantly different from the relevant 0 control (p < 0.01, Student's t test). Western blotting controls for expression of proteins in yeast are shown in Fig. S12.