Figure 4.

SNX6 knockdown impairs IGF1R signaling. A, a representative blot showing robust siRNA-mediated knockdown of SNX6. B, representative blot showing that SNX6 knockdown inhibited IGF1-induced phosphorylation of ERK1/2, whereas having no significant effect on IGF1-induced Akt phosphorylation. C, quantification of bands in B. D, a representative blot highlighting a similar trend for later treatment times. E, quantification of bands in D. One-way analysis of variance was performed followed by Bonferroni's Multiple Comparison Test, 0.0001 < ****, p < 0.001; ***, p < 0.001; *, p < 0.05. n = 3–4.