Figure 5.

SNX6 knockdown does not alter IGF1R internalization dynamics or cellular distribution. A, IGF1-induced IGF1R internalization was evaluated by flow cytometry. One-way analysis of variance revealed no significant differences in the extent of internalization as a result of SNX6 knockdown at any of the time points tested. All data were normalized to the 120 min values. n = 5. B, immunofluorescence was performed using an anti-HA antibody to visualize IGF1R distribution (red). This experiment was performed three times. Scale bar = 50 μm.