Table 1.
S. pombe and S. cerevisiae strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| FY435 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 | Ref. 43 |
| fep1Δ | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 fep1Δ::ura4+ | Ref. 43 |
| TMY1 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::KANr | Ref. 7 |
| TMY2 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::KANr | Ref. 7 |
| TMY17 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::loxP str3Δ::KANr | This study |
| VNY13 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::loxP str3Δ::KANr str3+:ade6+ | This study |
| VNY14 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::loxP str3Δ::KANr str3+-GFP:ade6+ | This study |
| VNY15 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::loxP str3Δ::KANr str3+-GFP:ade6+ shu1+-HA4:leu1+ | This study |
| VNY16 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::loxP str3Δ::KANr str3+Y530A/Y534A/S552A/Y557A:ade6+ | This study |
| VNY17 | h+ his7-366 leu1-32 ura4-Δ18 ade6-M210 hem1Δ::loxP shu1Δ::loxP str3Δ::KANr str3+Y530A/Y534A/S552A/Y557-GFP:ade6+ | This study |
| BY4741 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 | Ref. 44 |
| VNY18 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 hem1Δ::KANr | This study |
| VNY19 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 hem1Δ::KANr p415-GPD | This study |
| VNY20 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 hem1Δ::KANr p415-GPD-RBT5 | This study |
| VNY21 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 hem1Δ::KANr p415-GPD-str3+ | This study |
| VNY20 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 hem1Δ::KANr p415-GPD-str3+-GFP | This study |