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. 2018 Mar 9;293(17):6214–6229. doi: 10.1074/jbc.RA118.002372

Figure 2.

Figure 2.

DR induction upon DDC-induced liver injury is suppressed in Klf5-LKO mice. A, Kaplan-Meier survival curves of control (n = 49) and Klf5-LKO (n = 70) mice treated with DDC. B, serum ALP and T-BIL levels were measured in control and Klf5-LKO mice treated with DDC for 1 week (n = 5 mice) or 2 weeks (n = 6 mice). p values were calculated by Mann-Whitney U test. p values comparing the control and Klf5-LKO mice (DDC 2 weeks) are 0.00430 (ALP) and 0.0130 (T-BIL). C, immunostaining for CK19 (green) in the Klf5-LKO and control livers treated with DDC for 4 weeks shown with counterstaining for nuclei (blue). Scale bar, 100 μm. D, quantification of CK19+ areas in whole-liver sections. Data represent the mean ± S.D. n ≥ 4 mice for each time point. p values calculated by Mann-Whitney U test for each time point compare the control and Klf5-LKO mice and are as follows: 0.343 (DDC 0 week); 0.700 (DDC 1 week); 0.0207 (DDC 2 weeks); 0.0238 (DDC 3 weeks); and 0.0286 (DDC 4 weeks). E, immunostaining for EpCAM (green) and CK19 (red) in the Klf5-LKO and control livers treated with DDC for 4 weeks. Scale bar, 100 μm. F, 3D immunostaining for CK19 (green) in the Klf5-LKO and control livers treated with DDC for 4 weeks. Stacked images were obtained with confocal microscopy and used to reconstruct a 3D image using the IMARIS software. The image is shown in surface mode. Note that a CK19+ cell cluster separated from the biliary tree structure is observed in the Klf5-LKO liver (white arrow). Scale bar, 50 μm. Asterisks indicate that the p values are <0.05 (*, p < 0.06).