Table 1.
List of antibodies used in this study
| Antigen | Source/supplier | Host | Fixation | Dilution | Refs. |
|---|---|---|---|---|---|
| CD45a | Pharmingen, catalog no. 553081 | Rat | No fixation | 1:100 | |
| CK19 | In-house | Rabbit | PFA only or acetone and PFA | 1:2000 | 62 |
| EpCAM | Pharmingen, catalog no. 552370 | Rat | Acetone and PFA | 1:200 | |
| EpCAMa | In-house | Rat | No fixation | 1:100 | 60 |
| Ki67 | Invitrogen, catalog no. 14-5698-82 | Rabbit | PFA | 1:200 | |
| Klf5 | Generous gift from Drs. Ryozo Nagai (Jichi Medical University, Tochigi, Japan) and Ichiro Manabe (Chiba University, Chiba, Japan) (Km1784) | Rat | PFA | 1:300 | 63 |
| Prominin1 | Biolegend (San Diego), catalog no. 141207 | Rat | Acetone | 1:100 |
a These antibody were used for FACS. The others were used for immunostaining. PFA is paraformaldehyde.