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SDG8-CW interacts with histone H3 tail with specific recognition of sequence and preference of lysine methylation level. A, sequence of H3K4me1 mutant peptides. Residues substituted by alanine are in italics. B, ITC measurements of the interaction between SDG8-CW and H3K4me1 mutant peptides. C–G, ITC measurements of the interaction between SDG8-CW mutant and H3K4 peptides. H, KD -fold change of the binding of H3K4me peptides by WT and mutant SDG8-CW.
