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. 2018 Apr 30;15:127. doi: 10.1186/s12974-018-1158-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — IL-1β treatment decreased the cLTP-induced F-actin stabilization. FRAP analyses were performed using hippocampal neurons at 16–18 DIV that were transfected with Lifeact-GFP. a Illustration of FRAP setting. b Representative fluorescence images show spines of control group (-cLTP, top panel), cLTP group (middle panel), and cLTP+IL-1β group (bottom) during FRAP. Scale bar, 2 μm c Analysis of immobile fractions from data obtained in b. The mobile fraction and the immobile fraction (fi) were calculated as described in the “Methods” section. Data are mean ± SEM (n = 4, (*p < 0.05, ANOVA)