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. 2018 Apr 23;9:523. doi: 10.3389/fpls.2018.00523

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Copyright © 2018 Zhang, Li, Xing, Yan, Wang and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of OsYUC1 promotes crown root proliferation and auxin overproduction. (A) Massive crown root proliferation was observed when OsYUC1 was overexpressed (the right two plants). The overexpression lines initiated many crown roots from the shoots. The crown roots also had more and longer root hairs compared to the wild type plant (left). OsYUC1OX refers to OsYUC1 overexpression under the control of the strong maize UBIQUITIN promoter. OsYUC1OX-1 and OsYUC1OX-3 were two independent TO plants. Bar = 1 cm. (B) Relative transcript levels (folds) of OsYUC1 in the independent overexpression lines. OX refers to OsYUC1 overexpression. WT refers to WT. Note that expression levels in the OX lines were increased hundreds fold. (C) Comparison of IAA contents in whole seedlings of WT and OsYUC1 overexpression tines. The data are presented as mean ±SD (n = 3). ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 (Student’s t-test).