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. 1999 Oct;90(10):1104–1108. doi: 10.1111/j.1349-7006.1999.tb00684.x

Detection of Methylation Damage in DNA of Gastric Cancer Tissues Using 32P‐Postlabelling Assay

Dae‐Yong Kim 1,✉, Myung‐Haing Cho 2, Han‐Kwang Yang 3, Kari Hemminki 4, Jin‐Pok Kim 3, Ja‐June Jang 5, Rajiv Kumar 4
PMCID: PMC5925992  PMID: 10595739

Abstract

Gastric cancer is the most common cancer in Korea. The causes are still unknown but it has been speculated that gastric cancer is associated with consumption of foods rich in nitrates/nitrites or a high dietary intake of salt or pickled food. In the present study, we studied the level of alkylated DNA adducts formed in gastric cancer tissues in comparison with that in normal gastric mucosa. DNA was extracted from surgically removed gastric cancer tissues and patient‐matched normal gastric mucosa. The level of N7‐methyldeoxyguanosine was measured by 32P‐postlabelling assay after high performance liquid chromatography (HPLC) enrichment. We found that the level of N7‐methyldeoxyguanosine of gastric cancerous tissues was significantly higher than that of normal gastric mucosa (P=0.01685).

Keywords: Gastric cancer, DNA adduct, 32P‐Postlabelling, DNA damage

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REFERENCES

  • 1. ) Fuchs , C. S. and Mayer , R. J.Gastric carcinoma . N. Engl. J. Med. , 333 , 32 – 41 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 2. ) Cady , B. , Ross , R. and Silverman , M.Gastric adenocarcinoma. A disease in transition . Arch. Surg. , 124 , 303 – 308 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 3. ) Ahn , Y.‐O. , Park , B.‐J. , Yoo , K.‐Y. , Kim , N.‐K. , Heo , D.‐S. , Lee , J.‐K. , Ahn , H.‐S. , Kang , D.‐H. , Kim H. , Lee M.‐S. and Park , T.‐S.Incidence estimation of stomach cancer among Koreans . J. Korean Med. Sci. , 6 , 7 – 14 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4. ) Tatematsu , M. , Takahashi , M. , Hanaouchi , M. and Shirai , T.Effects in rats of sodium chloride on experimental gastric cancers induced by N‐methyl‐N‐nitro‐N‐nitroguanidine or 4‐nitroquinoline‐1‐oxide . J. Natl. Cancer Inst. , 55 , 101 – 106 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Ramon , J. M. , Serra , L. , Cerdo , C. and Oromi , J.Dietary factors and gastric cancer risk . Cancer , 71 , 1731 – 1735 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 6. ) Risch , H. A. , Jain , M. , Choi , N. W. , Fodor , J. G. , Pfeiffer , C. J. , Howe , G. R. , Harrison , L. W. , Craib , K. J. and Miller , A. B.Dietary factor and the incidence of cancer of the stomach . Am. J. Epidemiol. , 122 , 947 – 959 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 7. ) Hemminki , K.DNA adducts, mutations and cancer . Carcinogenesis , 14 , 2007 – 2012 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 8. ) Ohshima , H. and Bartsch , H.Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis . Mutat. Res. , 305 , 253 – 264 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 9. ) Cadet , J. and Weinfeld , M.Detecting DNA damage . Anal. Chem. , 65 , 675 – 682 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 10. ) Shields , P. G. and Harris , C. C.Molecular epidemiology and the genetics of environmental cancer . JAMA , 266 , 681 – 687 ( 1991. ). [PubMed] [Google Scholar]
  • 11. ) Shields , P. G. , Povey , A. C. , Wilson , V. L. , Weston , A. and Harris , C. C.Combined high‐performance liquid chromatography/32P‐postlabelling assay of N7‐methyldeoxyguanosine . Cancer Res. , 50 , 6580 – 6584 ( 1990. ). [PubMed] [Google Scholar]
  • 12. ) Haque , K. , Cooper , D. P. and Povey , A. C.Optimization of 32P‐postlabelling assay for the quantitation of O6‐methyl and N7‐methydeoxyguanosine‐3′‐monophosphate in human DNA . Carcinogenesis , 15 , 2485 – 2490 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 13. ) Zarbl , H. , Sukumar , S. , Arthur , A. V. , Martin‐Zance , D. and Barbacid , M.Direct mutagenesis of H‐ras‐1 oncogenes by N‐nitroso‐N‐methylurea during initiation of mammary carcinogenesis in rats . Nature , 315 , 382 – 385 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 14. ) Goth , R. and Rajewsky , M. F.Persistence of O6‐ethylgua‐nine in rat brain DNA: correlation with nervous system‐specific carcinogenesis by ethylnitrosourea . Proc. Natl. Acad. Sci. USA , 71 , 639 – 643 ( 1974. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15. ) Dumenco , L. L. , Allay , E. , Norton , K. and Gerson , S. L.The prevention of thymic lymphomas in transgenic mice by human O6‐alkylguanine‐DNA alkyltransferase . Science , 259 , 219 – 222 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 16. ) Poirier , M. C. and Beland , F. A.DNA adduct measurement and tumor incidence during chronic carcinogen exposure in animal models: implication for DNA adduct‐based human cancer risk assessment . Chem. Res. Toxicol. , 5 , 749 – 755 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 17. ) Ross , J. A. , Nelson , G. B. , Wilson , K. H. , Rabinowitz , J. R. , Galati , A. , Stoner , G. D. , Nesnow , S. and Mass , M. J.Adenomas induced by polycyclic aromatic hydrocarbons in strain A/J mouse lung correlate with time‐integrated DNA adduct levels . Cancer Res. , 55 , 1039 – 1044 ( 1995. ). [PubMed] [Google Scholar]
  • 18. ) Umbenhauer , D. , Wild , C. P. , Montesano , R. , Saffhill , R. , Boyle , J. M. , Huh , N. H. , Kirstein , U. , Thomale , J. , Rajewsky , M. F. and Lu , S. H.O6‐Methyldeoxyguanosine in oesophageal DNA among individuals at high risk of oesophageal cancer . Int. J. Cancer , 36 , 661 – 665 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 19. ) Lu , S. H. , Ohshima , H. , Fu , H. M. , Tian , Y. , Li , F. M. , Blettner , M. , Wahrendorf , J. and Bartsch , H.Urinary excretion of N‐nitrosamino acids and nitrate by inhabitants of high‐ and low‐risk areas for esophageal cancer in Northern China : endogenous formation of nitrosoproline and its inhibition by vitamin C . Cancer Res. , 46 , 1485 – 1491 ( 1986. ). [PubMed] [Google Scholar]
  • 20. ) Hall , C. N. , Badawi , A. F. , O'Connor , P. J. and Saffhill , R.The detection of alkylation damage in the DNA of human gastrointestinal tissues . Br. J. Cancer , 64 , 59 – 63 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21. ) Huh , N. H. , Satoh , M. S. , Shiga , J. , Rajewsky , M. F. and Kuroki , T.Immunoanalytical detection of O4‐ethylthymine in liver DNA of individuals with or without malignant tumors . Cancer Res. , 49 , 93 – 97 ( 1989. ). [PubMed] [Google Scholar]
  • 22. ) Wang , Y. , Ichiba , M. , Iyadomi , M. , Zhang , J. and Tomokuni , K.Effects of genetic polymorphism of metabolic enzymes, nutrition, and lifestyle factors on DNA adduct formation in lymphocytes . Ind. Health , 36 , 337 – 346 ( 1998. ). [DOI] [PubMed] [Google Scholar]

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