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Nigericin-mediated release of folate-Dy647-siRNA conjugate 5 from endosomes. (A) Structure of folate-Dy647-siRNA, 5. (B) Confocal fluorescence microscopy images showing the release of Dy647-labeled folate-siRNA (400 nM) from endosomes of KB cells in the absence (left panels) and presence (right panels) of folate-nigericin conjugate 2. The green arrows suggest “pluming” of contents as they stream away from the endosome.
