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. Author manuscript; available in PMC: 2018 Apr 30.
Published in final edited form as: Bioconjug Chem. 2018 Mar 2;29(4):1047–1059. doi: 10.1021/acs.bioconjchem.7b00714

Figure 7.

Figure 7

Nigericin-mediated escape of rhodamine from endosomes in FRα-expressing MDA-MB-231 cells and FRβ-expressing RAW264.7 cells. (A) MDA-MB-231 cells were treated with 10 nM folate rhodamine conjugate 4 in the absence (left panels) or presence of 100 nM folate-nigericin ester conjugate 2 (middle panels) or 100 nM folate-nigericin amide conjugate 3 (right panels). The green arrows identify sites of “pluming” of contents as entrapped rhodamine streams away from the endosome. (B) Nigericin-mediated escape of rhodamine from endosomes in FRβ-expressing RAW264.7 cells. Cells were treated with 10 nM folate rhodamine conjugate 4 in the absence (left panels) or presence (right panels) of 100 nM folate-nigericin ester conjugate 2.