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. Author manuscript; available in PMC: 2018 May 22.
Published in final edited form as: Dev Cell. 2017 May 22;41(4):438–449.e4. doi: 10.1016/j.devcel.2017.04.020

Figure 2. Ndc80 interacts with Ska3 in both prometaphase and metaphase.

Figure 2

(A) Representative images of Ska3-Ndc80 Proximity Ligation Assay (red), with additional immunostaining of Borealin (cyan) and tubulin (green). Top – prometaphase cells, middle – metaphase cells, bottom – control metaphase cells.

(B) Centromeres in prometaphase or metaphase cells, as identified by Borealin immunostaining, were scored based on the proximity to Ska3-Ndc80 PLA signal (mean ± SD; N > 100 centromeres from four cells). Cells with only Ska3 probe were used as a control. N/S – p > 0.05, * - p < 0.05, *** - p < 0.0001. (Student’s t-test)

(C) Centromeres in prometaphase or metaphase cells, as identified by Borealin immunostaining, scored based on the number of proximal Ska3-Ndc80 PLA signals (mean ± SD; N > 100 centromeres from four cells). N/S - p > 0.05, * - p < 0.05, *** – p < 0.0001. (Student’s t-test)