The experimental design is illustrated at the top of the figure. Subjects that received bicuculline (Bic) or its vehicle (Veh) are depicted as squares and circles, respectively. Groups given intermittent shock (Shk) or nothing (Unshk) are shown in black and white, respectively. (A) Mechanical thresholds on the treated leg after bicuculline administration (Post Drug) and 0, 1, 2, 3 hr after shock treatment (Post Shock). The left y-axis depicts linearized mechanical scores based on a transformation [log 10 (10,000g)] of the force required to bend the thinnest filament that elicited a paw withdraw and the right axis depicts the gram force equivalents. (B) Mechanical thresholds on the untreated leg. (C) Mechanical thresholds averaged across test leg. (D) The change from baseline scores for the treated leg. (E) The change from baseline scores for the untreated leg. (F) The change from baseline scores of averaged across test leg. The error bars depict ± SEM.