Figure 1.

SIRT1 and SIRT6 expression in lymphocytes. (A) Representative histogram of 3T3 cells (positive control) or (B) Lymphocytes from healthy subjects stained with anti-SIRT6 (black) and secondary antibody FITC anti-mouse as an isotype control (grey). (C) Comparison of SIRT6 expression in 3T3 and lymphocytes. (D) Western blot of representative SIRT6 protein expression level in peripheral blood mononuclear cells (PBMC) and positive controls. (E) Representative histogram of HeLa cells (positive control) or (F) Lymphocytes from healthy subjects stained with either rabbit anti-SIRT1 (black), goat anti-rabbit APC secondary antibody as isotype control (grey) or cells in the absence of antibody (dotted line). (G) Comparison of SIRT1 expression in HeLa and lymphocytes. (H) Western blot of representative SIRT1 protein expression level in PBMC and positive controls.