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. 2017 Dec 30;8(2):188–199. doi: 10.1016/j.apsb.2017.11.008

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Fig. 2 — SIRT6+, NAT1+ and NAT1+SIRT6+ expression in lymphocytes. Peripheral blood mononuclear cells (PBMC) were stained as described in Section materials and Methods. (A) Dot plots of FCS and SSC where the lymphocyte gate was selected. (B) Histogram of isotype control coupled to FITC in the lymphocyte gate (P1). (C) Histogram of anti-SIRT6 (primary antibody) and FITC anti-mouse (secondary antibody) in the lymphocyte gate (P1). (D) Histogram of isotype control coupled to APC in the lymphocyte gate (P1). (E) Histogram of anti-NAT1 (primary antibody) and APC anti-rabbit (secondary antibody) in the lymphocyte gate. (F) Dot plot of isotype control-FITC in the lymphocyte gate. (G) Dot plot of anti-SIRT6 (primary antibody) and FITC anti-mouse (secondary antibody) in the lymphocyte gate. (H) Dot plot of isotype control-APC in the lymphocyte gate. (I) Dot plot of anti-NAT1 (primary antibody) and APC anti-rabbit (secondary antibody) in the lymphocyte gate. (J) Representative dot plot of double staining with anti-SIRT6-FITC and anti-NAT1-APC in the lymphocyte gate.

Fig. 2 — SIRT6+, NAT1+ and NAT1+SIRT6+ expression in lymphocytes. Peripheral blood mononuclear cells (PBMC) were stained as described in Section materials and Methods. (A) Dot plots of FCS and SSC where the lymphocyte gate was selected. (B) Histogram of isotype control coupled to FITC in the lymphocyte gate (P1). (C) Histogram of anti-SIRT6 (primary antibody) and FITC anti-mouse (secondary antibody) in the lymphocyte gate (P1). (D) Histogram of isotype control coupled to APC in the lymphocyte gate (P1). (E) Histogram of anti-NAT1 (primary antibody) and APC anti-rabbit (secondary antibody) in the lymphocyte gate. (F) Dot plot of isotype control-FITC in the lymphocyte gate. (G) Dot plot of anti-SIRT6 (primary antibody) and FITC anti-mouse (secondary antibody) in the lymphocyte gate. (H) Dot plot of isotype control-APC in the lymphocyte gate. (I) Dot plot of anti-NAT1 (primary antibody) and APC anti-rabbit (secondary antibody) in the lymphocyte gate. (J) Representative dot plot of double staining with anti-SIRT6-FITC and anti-NAT1-APC in the lymphocyte gate.