Figure 8.

A, Expression of PMP22 during seedling development. Total protein was extracted from an equal number (based on dry weight at sowing) of Arabidopsis seedlings at the indicated number of hours postimbibition. Protein samples were separated by SDS-PAGE and probed with affinity-purified anti-PMP22 antibodies, anti-castor bean isocitrate lyase antibodies (ICL), or affinity-purified anti-spinach glycolate oxidase antibodies (GO). B, Effects of light on expression of PMP22 in tissue cultures. Arabidopsis suspension-cultured cells were grown in either continuous light (lane 1) or total dark (lane 2), subcultured, and cultured for a further 5 d under the same conditions. Total protein was extracted from these samples and subjected to SDS-PAGE and immunoblotting with affinity-purified anti-PMP22 antibodies.