Abstract
The influence of 1′‐acetoxychavicol acetate (ACA) during the initiation stage was investigated in the N‐nitrosobis(2‐oxopropyl)amine (BOP)‐initiated hamster tumorigenesis model. Ninety male 5‐week‐old hamsters were divided into three groups, each consisting of 30 animals, and s.c. injected with 20 mg/kg of BOP twice with a one‐week interval. Groups 1 through 3 were fed diet supplemented with ACA at concentrations of 500, 100 and 0 ppm, respectively, for 3 weeks starting one week before the first carcinogen application. At the termination of experimental week 54, the total incidence and multiplicity of cholangiocellular adenomas and carcinomas in group 1 (17.9% and 0.3±0.9) were significantly (P< 0.05 and P< 0.01) decreased as compared to the group 3 values (50.0% and 0.7±0.8). The ACA treatments also showed a tendency to reduce the development of preneoplastic lesions in the pancreas, a main target organ of BOP, although this was not statistically significant. Our results thus indicate that ACA exerts an inhibitory effect on BOP‐induced cholangiocarcinogenesis in hamsters. Taken together with previous findings of inhibited colon, oral and skin carcinogenesis in rats and mice, they suggest that ACA is a candidate chemopreventive agent with a wide spectrum of activity.
Keywords: 1′‐Acetoxychavicol acetate, N‐Nitrosobis(2‐oxopropyl)amine, Cholangiocarcinogenesis, Hamster
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