Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Separation of Fd- and NADH-GOGAT by anion-exchange column chromatography. The crude extracts of wild-type, HOF12, HOB12, and HOD12 strains of P. boryanum were applied to a Resource-Q column (0.64 × 3 cm) and eluted with a linear gradient of NaCl at a flow rate of 1.0 mL/min as described in Methods. Elution profiles of Fd- and NADH-GOGAT were monitored by assay of their activities using 10 μm Fd and 1 mm NADH as electron donors, respectively.
