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Fd (40 μm)-, NADH (1 mm)-, and NADPH (1 mm)-dependent GOGAT activities in crude extracts of the control strain and GOGAT-disrupted strains of P. boryanum
| Strain | Fd | NADH | NADPH |
|---|---|---|---|
| nmol Glu min−1 mg−1 protein | |||
| YFD1 (control)a | 45 ± 15 | 42 ± 11 | <1b |
| HOF12 (glsF mutant) | <1 | 48 ± 10 | <1 |
| HOB12 (gltB mutant) | 44 ± 7 | <1 | <1 |
| HOD12 (gltD mutant) | 44 ± 5 | <1 | <1 |
Control strain with the kanamycin cartridge inserted in a neutral site of the genome.
<1, Negligible values (below the detection limit).
