Figure 4. CD38⁺ M-MDSCs from the peripheral blood of CRC patients are immunosuppressive.

(A) Immunosuppressive capacity of M-MDSCs. M-MDSCs (CD14⁺HLA-DR^lo) and monocytes (CD14⁺HLA-DR^hi) were isolated from PBMCs, and immunosuppressive activity of these cell populations was tested in 3-way allogenic MLR. Gray dots represent individual values (averaged triplicate measures at 1:1 MDSC/monocyte to T cell ratio; black squares, mean values). Statistical data are presented in Table 5. (B) A representative result of MLR with M-MDSCs and monocytes from a single CRC patient. Individual values are plotted as gray squares (Monocyte) or black circles (M-MSDC); horizontal bars represent mean values; error bars indicate SEM; P = 0.02 (1:1), P = 0.01 (1:3), P = 0.009 (1:9).