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. 2018 Mar 22;3(6):e98738. doi: 10.1172/jci.insight.98738

Figure 7. Verteporfin reduces YAP transcriptional activity and phosphorylation of S6 in absence of light.

Figure 7

HBEC3s transduced with GFP, YAP (WT), or YAP (S127A) lentiviral vectors were treated with either vehicle (DMSO) or verteporfin (2.0 or 10.0 μg/ml) for 48 hours (n = 3). (A) Western blot for YAP and p-S6 following a verteporfin dose curve in complete darkness show reduced total YAP and p-S6. (B) Western blot analysis of YAP and p-S6 following verteporfin treatment for 48 hours with MHY1485 treatment for the final 24 hours shows increased YAP after mTOR activation. Addition of MG132 for the final 24 hours of verteporfin treatment protects YAP from degradation caused by verteporfin. (C) Yap, JUB, CTGF, AXL, PLAU, and WNT7B RNAs were assessed in experiments performed in complete darkness (n = 4 transfections). RNA expression is normalized to DMSO-treated cells. *P < 0.05, determined by ANOVA. HBEC3s, hTERT/CDK4–immortalized human bronchiolar epithelial cells.