Pressure curves were run on third-order mesenteric arteries isolated from C57Bl/6 mice in the presence or absence of carbenoxolone (CBX; 50 μM; A–C), spironolactone (80 μM; D–F), or mesenteric arteries isolated from EC Panx1 (fl/fl and Δ/Δ) mice (G–I). Percent myogenic tone, active diameter, and passive diameter were analyzed for each group. Control (CBX), n = 5; CBX, n = 3; control (spironolactone), n = 3; spironolactone, n = 3; EC Panx1 fl/fl, n = 4; and EC Panx1 Δ/Δ, n = 6 mice. *P < 0.05. Two-way ANOVA/Sidak’s multiple comparisons post hoc test. Telemetry was used to collect average day and night mean arterial pressure (J) and heart rate (K) from EC Panx1 fl/fl and EC Panx1 Δ/Δ mice. No significant differences were found using Student’s t test.