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. 2018 Mar 22;3(6):e98459. doi: 10.1172/jci.insight.98459

Figure 2. HETE-PLs enhance thrombin generation in the absence of FIX or FXI, or in the absence of tissue factor pathway inhibitor (TFPI) and FVIII combined.

Figure 2

(A and B) Thrombin generation was initiated by addition of liposomes to plasma deficient in FIX, as described in Methods. Liposomes were generated as described in Methods, and 10% 1-stearoyl-2-arachidonyl-PE (SAPE) was replaced with 10% 15-HETE-PE where indicated. (C and D) Thrombin generation was initiated by addition of liposomes to plasma deficient in FXI, as described in Methods. Liposomes were generated as described in Methods, and 10% SAPE was replaced with 10% 15-HETE-PE where indicated. (A–D) n = 3, mean ± SEM, *P < 0.05. A representative trace is shown for each. (E–H) Thrombin generation was initiated by direct addition of liposomes to plasma as described in Methods, at 10% HETE-PE, and measured in the absence (E and G) or presence (F and H) of 100 nmol/l anti-TFPI antibody preincubated for 15 minutes, in either normal (E and F) of FVIII-deficient plasma (G and H). (I–L) Thrombin generation was initiated by direct addition of liposomes to plasma as described in Methods, at 10% HETE-PC, and measured in the absence (I and K) or presence (J and L) of 80 nmol/l anti-TFPI antibody preincubated for 15 minutes, in either normal (I and J) of FVIII-deficient plasma (K and L). Data is shown as Tukey box plots, with 1-way ANOVA with Tukey post hoc multicomparison test. *P < 0.05.