Figure 2. Absence of ANGPTL4 in adipose tissue (AT) enhances plasma TAG clearance and tissue lipid uptake.

(A) mRNA expression of Angptl4 in brown (BAT) and white adipose (WAT) tissues from WT, AT-specific Angptl4-knockout (Ad-KO), and Angptl4^–/– mice (n = 4). (B) Fed and fasted plasma triacylglycerol (TAG) levels in WT and Ad-KO mice (n = 8). (C and D) Total cholesterol (TC) and HDL-C levels in plasma of WT and Ad-KO mice fasted overnight (n = 7–8). (E) Oral lipid tolerance test showing the clearance of TAGs from the plasma of fasted WT and Ad-KO mice that were fasted for 4 hours followed by an oral gavage of olive oil (n = 8). (F) Radioactivity incorporation in indicated tissues after 2 hours of oral gavage of [³H]-labeled triolein in WT or Ad-KO mice fasted for 6 hours (n = 3). (G) Lipoprotein lipase (LPL) activity in the indicated tissues isolated from WT and Ad-KO mice. For plasma LPL activity, blood was drawn 5 minutes after heparin injection (n = 3). (H) Plasma TAG levels from WT and Ad-KO mice fasted overnight and treated with the LPL inhibitor poloxamer 407 to inhibit lipolysis of TAG-rich lipoprotein particles (n = 3). All data represent the mean ± SEM. *P ≤ 0.05 comparing Ad-KO with WT mice using unpaired t test. NS, not significant.