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. 2018 Apr 27;12:997–1008. doi: 10.2147/DDDT.S151029

Figure 6.

Figure 6

Effects of 4-amino-2-trifluoromethyl-phenyl retinate (ATPR) on proliferation of MCF-7 cells after silencing of the FABP5 gene.

Notes: (A) Immunofluorescence staining of MCF-7 cells confirms a significant reduction in FABP5 expression in the FABP5-siRNA group. Representative images were obtained using a 200× objective. (B) An MTT assay was performed on transfected MCF-7 cells that were untreated or treated with 1 × 10−6 mol/L ATPR for 24, 48, and 72 h. *P < 0.05. For 48 h, ATPR(−) siRNA(−) vs ATPR(+) siRNA(−) (one-way ANOVA, P = 0.012), ATPR(−) FABP5 siRNA(+) vs ATPR(+) FABP5 siRNA(+) (one-way ANOVA, P = 0.167), ATPR(−) siRNA(−) vs ATPR(−) FABP5 siRNA(+) (one-way ANOVA, P = 0.258). For 72 h, ATPR(−) siRNA(−) vs ATPR(+) siRNA(−) (one-way ANOVA, P = 0.018), ATPR(−) siRNA(−) vs ATPR(−) FABP5 siRNA(+) (one-way ANOVA, P = 0.019), ATPR(+) siRNA(−) vs ATPR(+) FABP5 siRNA(+) (one-way ANOVA, P = 0.943), ATPR(+) siRNA(−) and ATPR(+) FABP5 siRNA(+) (one-way ANOVA, P = 0.998). “NS” is not significant.